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RESEARCH TECHNICIAN III - Human Vaccine Institute

Req ID:  11491
Date:  Nov 6, 2019

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER

School of Medicine:


Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.


Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.


Research Technician III



  • Work as part of a multi-disciplinary research team to characterize human B cell responses utilizing flow cytometry, reverse transcription PCR, ELISA, Biolayer interferometry, cloning, cell culture, antibody expression and purification.
  • Operate and maintain DNA sequencer and liquid handling automated workstations.
  • Understand and comply with all safety standards and procedures to insure a safe working environment for all laboratory staff.
  • Design, execute, analyze and archive experiments in a manner that is scientifically justifiable, reliable and understandable to others.
  • Keep lab equipment, chemicals reagents and supplies in excellent order.  Perform routine lab maintenance, cleaning, ordering and organizing.
  • Learn and develop new lab techniques and teach them to other lab staff. 
  • Collaborate with other staff, your supervisor, and Principal Investigator(s) to review experimental results and develop new research plans; assist in the training of new personnel.
  • Perform other duties as requested by your supervisor, Principal Investigator or senior Institute Leadership.



  • Bachelor’s degree in biology with 2 years of research experience, a 2 year master’s degree or an equivalent combination of relevant education and experience.
  • Solid mathematical skills as it relates to the calculation of weights, volumes and solution concentrations.
  • Familiarity with cellular and molecular biology techniques
  • Must be detail oriented with the ability to perform repetitive, reproducible assays according to a carefully designed plan/protocol.
  • Computer literate as it relates to Microsoft Word, PowerPoint, Excel, and other graphing/analytical software with a willingness to learn new systems/skills.



  • Perform laboratory work at Biosafety Level 2 and 3.
  • Comply with vaccination requirements when necessary
  • Complete US Department of Justice screening for Select Agent registration
  • Complete Select Agent registration at Tier 1
  • Achieve external certification for independent work at Biosafety 3



Minimum Qualifications


Work generally requires skills acquired through the completion of a bachelor's degree in botany, biology, zoology, psychology or other science related scientific field program.



Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE


Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh