RESEARCH TECHNICIAN III

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

Be You.

The Asokan lab (www.asokanlab.org) is affiliated with the Duke Department of Surgery, Molecular Genetics & Microbiology & Biomedical Engineering and is focused on the development and evaluation of novel gene therapy vectors. We are currently seeking to hire a motivated candidate for a Research Technician III position to assist with in-vivo experimentation and animal husbandry techniques in the lab. A competitive salary and benefits package will be provided according to the Duke University School of Medicine guidelines.

Minimum Requirements:

• Work generally requires skills acquired through the completion of a bachelor's degree in botany, biology, zoology, psychology or other science related scientific field program.
• Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.
• OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Preferred Qualifications:

• Bachelor’s degree in biology, chemistry, genetics, microbiology or other related scientific fields.
• Two years of research experience involving mouse work.
• Detail-oriented, very well organized, and approach laboratory procedures with critical thinking.
• Strong initiative and eagerness to learn.
• Outstanding problem-solving skills.
• Must adhere to strict safety requirements.
• Ability to work and communicate effectively in a dynamic group environment. 

Other Requirements:

This position is onsite. The work is performed on-site or at a designated assignment location.

Be Bold.

The candidate will work closely with lab members including postdoctoral fellows, graduate students, research technicians, and is expected to independently perform daily activities which include, but may not be limited to:

• CRISPR assays and NGS sample prep
• AAV preparation, quality control and titering, maintain suspension tissue culture systems of up to 20L, operate tangential flow filtration and chromatography systems to purify AAV virus, and analyze sequencing data to ensure quality control between different virus preparations.
• Tissue culture maintenance, DNA/RNA extraction and cloning DNA constructs in bacterial systems.
• Perform analytical techniques on vector preparations (qPCR, ELISA, protein gels etc).
• Obtain, prepare, mount and stain tissue, blood and other substances for microscopic examination.
• Perform recombinant protein expression from bacteria.
• Ensure validity and reliability of data and maintain accurate records.
• From time-to-time support additional high-priority projects to meet deadlines.
• Attend and participate in relevant meetings, training, and development sessions.

• Determine and setup instruments and operate laboratory equipment required for specific tests.

• Use and maintain lab equipment in accordance with university’s health & safety guidelines.

• Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments.
• Review work of lower-level laboratory personnel within designated areas and assist in training new personnel.

Choose Duke.

Join our award-winning team and be part of an inclusive culture that is committed to providing outstanding patient care, training tomorrow’s leaders, and conducting innovative research. You'll also benefit from Duke's competitive benefits package, including health insurance plans, generous paid time off, retirement programs with employer contributions, tuition assistance for employees and their children, and more.

Screening of Applications: Screening continues until the position is filled.

Minimum Qualifications

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexualorientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve of this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Anticipated Pay Range:

Duke University provides an annual base salary range for this position as USD $22.61 to USD $34.27. Duke University considers factors such as (but not limited to) scope and responsibilities of the position; candidate's work experience, education/training, and key skills; internal peer equity; as well as market and organizationalconsiderations when extending an offer.

Your total compensation goes beyond the dollars on your paycheck. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array offamily- friendly and cultural programs to eligible team members. Learn more at: https://hr.duke.edu/benefits/

Essential Physical Job Functions:

Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Education

Work generally requires skills acquired through the completion of a bachelor's degree in botany, biology, zoology, psychology or other science related scientific field program.

Experience

Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Degrees, Licensures, Certifications

Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE