RESEARCH TECHNICIAN III

Occupational Summary 

We are seeking a research technician III to join our childhood cancer translational research team. We study the pediatric skeletal muscle tumor rhabdomyosarcoma, and we are leading several national research efforts to develop new therapies for this difficult-to-treat cancer. Focus is on how mutations in coding genes - and noncoding genes such as long-noncoding RNAs - corrupt normal developmental signaling pathways (Notch, Hippo) and might reveal novel therapeutic vulnerabilities. We collaborate with investigators within and beyond Duke. We use biochemical, cell biology, molecular biology, genomic, epigenomic, and mouse model approaches. We value teamwork, creativity, and resilience. Career development is tailored to the candidate’s goals. See our lab’s website https://sites.duke.edu/corinnelinardiclab/.

Work Performed 

· Design and execution of standard in vitro assays ongoing in the lab (transient or stable expression of cDNA/shRNAs/CRISPR, mutagenesis, IPs, immunoblotting, nucleic acid extractions, PCR, phenotype-based cell assays including proliferation, apoptosis, etc) 

· Design and implementation of in vitro screens with support from Duke Core Facilities 

· Participation in in vivo murine pre-clinical studies and tumor tissue analyses including immunohistochemistry and preparation for next-gen sequencing 

· Application of statistically sound analyses 

· Participating in drafting of LOIs, grant applications, abstracts, posters, and manuscripts 

· Participating and presenting in domestic/international childhood cancer think thank meetings 

· Functioning as a resource for early stage scientists (novice graduate students and technicians) 

· Flexing one’s intellectual muscles to think out-of-the box for this difficult-to-cure cancer 

Required Qualifications at this Level 

· Bachelor’s in the life sciences with at least three years of experience, or an equivalent combination of relevant education and/or experience such as a Master’s degree. 

· Candidates with a record of publication in peer-reviewed journals and expertise in cancer biology are encouraged to apply. 

· Ability to perform standard molecular biology, biochemistry, and cell culture techniques; mouse experience is a plus but we can also teach you this. 

· Qualifying competencies include excellent oral and written communication skills. 

· Excellent organizational skills, creativity, ability to work independently and as part of a team. 

· Preference will be given to highly self-motivated individuals interested in career advancement and growth opportunities 

How to apply 

Candidates should send their CV, a brief letter of research interest and a list of references to: Corinne Linardic, MD PhD, corinne.linardic@duke.edu

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