RESEARCH TECHNICIAN II

This position is Grant Funded

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Research Technician II

Moody Discovery Laboratory

OVERVIEW:

The Moody Discovery Laboratory is looking for a research technician II to assist our efforts on a Collaborative Influenza Vaccine Innovation Centers CIVICs project focused on improving the seasonal influenza vaccine. This position is lab based and will include running immunological assays and assisting with protein production related to influenza research.  The Moody lab is part of DHVI which has a mission of performing research against infectious diseases that impact global health.  Our lab is located at RTP.  The Research Technician II will report directly to the Senior Research Laboratory staff.  Applicants are required to have an undergraduate degree and prior research experience.

RESPONSIBILITIES:

1. Assist with protein production work flow tasks
   1. Collaborate with protein production team to fulfill protein requests
   2. Methods can include DNA transformation/ production/ purification, transfection of mammalian or insect cells, purification of proteins and validation of proteins.
   3. Documentation of methods in data base and production of certificates of testing

2. Perform immunoassays for Influenza studies. 
   1. Assays may include but not limited to ELISA, ELLA and Blocking ELISA.
   2. Responsibilities will include planning, collecting and interpreting data following SOP’s.

3. Contribute to work flow to characterize the B cell repertoire induced by influenza virus infection assisting with protein conjugations, flow cytometry, PCR, and small-scale transfections working closely with post doc or senior research scientist.

4. Collaborate with computer analysists in the lab to develop software for Moody lab documentation, reporting and sample storage.  Experience with electronic lab notebooks.

5. Contribute to general lab maintance activities
   1. Including but not limited to lab organization, ordering, maintaining lab inventory, updating SOP’s, updating assay cost analysis report, and lab safety.
   2. Present data to Moody Laboratory and program project teams in both written and oral updates.  Prepare reports as needed for project.

6. Perform other duties as assigned by the PI or senior lab staff.

REQUIREMENTS:

Minimum Bachelor’s level degree in biology or related field.  Research experience.  Must have proven organizational skills, communication skills, and proficiency in Microsoft Excel, Word and PowerPoint.  Experience with FlowJo andalysis software and GraphPad Prism software highly preferred.

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