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Duke University and Duke University Health System require all students, faculty and staff to receive the COVID-19 and influenza vaccines.  After you have officially accepted a position at Duke, you may request a religious or medical accommodation.


Work Arrangement: 
Requisition Number:  195216
Regular or Temporary:  Regular

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER
Date:  Jul 28, 2022

This Position is Grant Funded

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.



The Duke Human Vaccine Institute (DHVI), an international leader in the fight against infectious diseases, is seeking a highly motivated individual for the position of Research Technician II. Investigators at the DHVI conduct basic and translational research to develop novel vaccines, therapeutics and diagnostics.


The Duke Regional Biocontainment Laboratory (RBL) is a division of the DHVI and supports a wide portfolio of research programs. The RBL has three collaborative core units: Immunology, Virology, and Microbiology. The Research Technician II will work with the RBL Immunology team and Duke Investigators to prepare specimens for flow cytometry, cell sorting, bulk and single-cell RNA sequencing and other assays. In addition, the technician will collect and interpret data and perform general laboratory maintenance and inventory management.


The ideal candidate will have a Bachelor’s degree in biological science or a related field.  Previous laboratory experience in molecular biology or immunology is highly preferred. Experience with PCR, sequencing library preparation and/or flow cytometry is an advantage but not required.



  • Prepare samples for and perform assays including, but not limited to, bulk and single-cell RNA sequencing, flow cytometry, multiplex bead arrays (Luminex platform), and real-time PCR.
  • Confer with Principal Investigator / Immunology Unit Manager to review work assignments and develop plans for experiments; make minor modifications to existing laboratory procedures and techniques to meet the needs of the particular study as required.
  • General laboratory maintenance including but not limited to: waste processing, laboratory organization, preparation of solutions, ordering/preparation/stocking of consumables and reagents as needed, maintaining accurate inventory of refrigerator and freezer contents.
  • Coordinate equipment maintenance performed by outside vendors.
  • Attend all required training.
  • Manage and complete all assigned tasks/projects in accordance with standardized operating procedures and in full compliance with safety protocols.
  • Maintain organized records of research activities according to standardized record keeping in real time.
  • Collect and organize raw data into reports for interpretation and analysis.
  • Perform additional duties as requested by Unit Manager and RBL/DHVI Leadership.



Must be detail-oriented, well organized with strong communication skills and able to work in a team environment.


The successful applicant must be eligible and willing to:

  • Complete necessary training and certification for independent work at Biological Safety Level 3 (BSL3)
  • Work with infectious biological agents (viruses and bacteria) handled at BSL2 and BSL3.
  • Comply with vaccination requirements.
  • Complete Tier 1 Select Agent registration.

Minimum Qualifications



Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.




None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE


Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh