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RESEARCH TECHNICIAN II

Work Arrangement: 
Requisition Number:  172669
Regular or Temporary:  Regular
Location: 

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER
Date:  May 31, 2022

School of Medicine:

 

Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

 

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

 

Research Technician II

Medicine/Medical Oncology/Melanoma Disease Group

The primary purpose of this position is to provide significant research support for the development of a translational research program in cancer immunotherapy.  The primary responsibilities for this position will involve overseeing the management of tumor immunotherapy protocols in murine tumor models as well as the processing and organization of human tumor tissue specimens for downstream studies.  This position will require experience in handling and basic care of mice including but not limited to subcutaneous injection of tumor lines, orthotopic injection of tumor lines, and delivery of therapeutic agents via intraperitoneal injection and oral gavage.  In addition, individuals in this position will carry out tumor cell line maintenance, Western blot analysis, RNA isolation procedures, qrt-PCR assays, tissue preparation for immunohistochemistry and immunofluorescence assays, and the isolation of cells for various. downstream immunologic assays. 

•             Maintaining and passaging murine tumor cell lines for subcutaneous implantation in syngeneic mice

•             Monitoring in vivo tumor growth by caliper measurements as well as by in vivo immunofluorescence imaging

•             Treatment of tumor-bearing mice with various therapeutic agents involving both intraperitoneal and oral gavage delivery

•             Collection of human melanoma specimens and downstream RNA/DNA isolation and tissue processing; organization of human-derived specimens in a tissue databank

•             Performing immunohistochemistry and immunofluorescence studies on both human and murine tumor tissues

•             Cell harvesting and purification from murine tumor tissues for immunologic studies including multi-parameter flow cytometry

•             Performing Western blot assays, qrt-PCR assays, and ELISAs

•             Documents protocol information and generated data in laboratory notebook

•             Assists with general laboratory maintenance duties including assisting with laboratory inventory, placing orders, and verifying their receipt; including preparation of lab solutions

•             Participates in lab meeting discussions




     
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Minimum Qualifications

 

Education

Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

 

Experience

 

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

 

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

 

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

 

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

 


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh