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RESEARCH TECHNICIAN II - Human Vaccine Institute

Req ID:  11487
Date:  Nov 6, 2019

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER

School of Medicine:


Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.


Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.


RESEARCH TECHNICAN II (Protein Production Facility)





Perform a variety of experimental duties in the Protein Production Facility under GxP conditions. 


Perform routine production and quality control of plasmids to be used in the transfection of mammalian and insect cells; follow laboratory SOPs and maintain detailed records of all work performed; transform bacterial cells and prepare (glycerol) stocks for plasmid production; produce plasmids for the use in transfection of mammalian cells; compare plasmid sequence homology; maintain an extensive but concise inventory of all plasmids and glycerol stocks.  Perform routine production of proteins and antibodies through transient transfection of mammalian and insect cells in compliance with good documentation practices; transfect cells, harvest, concentrate and purify proteins and antibodies as required to fulfill requests made to the PPF. Assist in maintaining and tracking an inventory of all proteins and antibodies produced.


  • Mammalian and insect cell culture; cells are to be used for transfection/ infection work; perform transfections/infections as required.
  • Respond to alerts and alarms from laboratory monitoring equipment.
  • Purify proteins derived from cell supernatant.  Purify and quantify proteins.
  • Protein analysis including FPLC, gel electrophoresis and western blots.
  • Setup, operate, repair, clean and maintain laboratory instruments and equipment.
  • Maintain sufficient inventory of material, supplies and equipment for performance of duties.
  • Maintain a clean, neat and orderly laboratory environment.
  • Prepare culture media, chemicals, reagents and solutions.
  • Maintain detailed written and/or electronic records in accordance with Protein Production Facility and DHVI policies.  Implement standard operating procedures (SOPs) as required and participate in the review/maintenance of SOPs in the lab.
  • Review laboratory methods, manuals, scientific journals, abstracts, and other literature for information applicable to experiments.
  • Perform other duties as requested by the Protein Production Facility or senior lab staff.


Education, Skills, & Experience Requirements


  • Prior experience working with baculovirus and culturing insect cells is preferred.
  • Bachelor’s degree in biology or a related field.
  • Must be detail-oriented, well organized and approach procedures with critical thinking of the technical aspects with strong communication skills and ability to work in an interactive team environment. 


Minimum Qualifications



Work generally requires abachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.




None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE


Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh