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Req ID:  10557
Date:  Oct 22, 2019

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER

School of Medicine:


Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.


Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.





  • Execute cultures of primary cells with impeccable sterile techniques and adherence to safety protocols.
  • Proficiently manipulate clinical samples (whole blood, biopsies, PBMCs, etc.), primary cells and established cell lines, culture supernatants and stocks of infectious agents.
  • Prepare culture media, reagents and solutions according to specifications.
  • Isolate primary cells using fluorescent-activated or magnetic-activated cell sorting from specimens collected from individuals, non-human primates and other small animals.
  • Perform functional high-throughput screenings (HTS) of biological samples (e.g. serum), cell culture supernatants and purified antibodies (e.g. ELISA, neutralization assays, etc.).
  • Coordinate the acquisition of reagents (both internally and externally) needed to complete assigned experiments.
  • Operate and maintain laboratory equipment, including complex automation systems.
  • Maintain laboratory workspace organized, clean and compliant with safety regulations. 
  • Perform DNA and RNA extraction, RT-PCR, nested PCR and overlapping PCR.
  • Produce and purify monoclonal antibodies from transiently transfected cell cultures.
  • Document, compile, analyze, and draw preliminary conclusions from experimental results. 
  • Maintain detailed documentation of executed work, ensure data integrity and accessibility, and generate timely, concise and precise reports.
  • Confer with PI and laboratory personnel to review work assignments and develop experimental plans
  • Adhere to BSL-2 and BSL-3 working conditions and safety regulations.
  • Perform other duties as requested by the PI. 


Work Schedule


You must be willing and available to work flexible hours which may include later start and end times with the understanding that work hours may need to be modified to ensure a project/task has reached a logical and acceptable stopping point to maintain the integrity of the science.


PREFERRED Education, Skills, & Experience Requirements


  • Bachelor’s degree in a related field.
  • Experience in handling cell/tissue cultures with strong record of performing sterile techniques
  • Experience in performing biochemical and immunological measurements of biological variables (e.g. ELISA, bead-based multiplex assays, flow cytometry).
  • Experience in performing RNA extraction, RT and PCR.
  • Experience in protein purification and identification techniques.
  • Willing to work with human specimens
  • Must be detail-oriented, focused, well organized and approach procedures with critical thinking and strong experimental record keeping.
  • Must be able to manage multiple and rapidly changing priorities with firm deadlines in a highly dynamic and competitive environment, execute experiments and generate deliverables in a timely fashion.
  • Must be able to work effectively and follow procedures independently and in a team environment as required.
  • Must have excellent communication skills. 




Minimum Qualifications




Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.




None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE


Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh