RESEARCH TECHNICIAN II

Be Bold

The Department of Radiation Oncology at Duke University is seeking a motivated research technician to assist in our efforts to understand fundamental mechanisms driving the development and progression of cancer, ultimately aiming to find better treatments for patients with cancer. This position is intended to provide experimental support by working in the laboratory of physician scientist Dr. Christine Eyler (https://radonc.duke.edu/research/eyler-lab). 

Research focuses on the following broad topics of cell and cancer biology: (1) probing mechanisms of treatment resistance and radiation response, (2) designing and conducting genetic and pharmacologic screens to identify novel cancer therapeutic strategies in cellular and/or organoid model systems, (3) implementation of advanced functional genomics techniques and 3D chromatin profiling to determine the spatial and functional requirements of enhancers, and (4) tissue processing and molecular analysis of patient-derived clinical samples. The Eyler laboratory possesses a rich environment including numerous research technicians, and students, and with numerous close collaborators. 

Competitive applicants will have received an undergraduate degree by the start of employment, will have prior experience in lab research, will be intellectually passionate and able to display scientific initiative. Candidates will gain the most from the experience if they are able to stay for more than 1 year of time, ideally 2 years or more. 

Be You.

Duties may include some or all of the following: 

· Maintain an organized and complete lab notebook of experiments that are performed. 

· Propagate cells in cell culture and perform in vitro viability assays and clonogenic assays. 

· Use human-derived specimens to establish and maintain cancer organoid culture models 

· Perform PCR, RT-PCR and Western blots to characterize cell lines, tumor tissues, and mouse tissues. 

· Learn and implement advanced techniques like high throughput microscopy, confocal microscopy, flow cytometry. 

· Learn and implement techniques for next generation sequencing library preparation, including analysis for motivated/interested candidates 

· Though much of the work is conducted in cell culture lines, some experiments will involve use of mouse models. Ideal candidates will be comfortable with learning these techniques. 

· Participate in a regular “call” schedule for retrieval, processing, and analysis of patient-derived clinical samples on a prospective clinical trial 

· Meet or communicate by e-mail with your supervisor on a regular basis to plan experiments, troubleshoot problems, and discuss results. 

· Participate in regular lab meetings to discuss results or articles in the literature. 

· Assist in recruiting new lab members by informing them about the lab and your research. 

· Help maintain reagents or equipment in the laboratory with specific jobs (which might include tasks like making cell dissociation buffer, 70% ethanol, etc.). 

· Help provide a collegial environment for the laboratory. 

· Set up instruments, material and apparatus and operate standard laboratory equipment required for specific tests. 

· Participate in laboratory logistical management, potentially including coordination of inspections, ordering, equipment repair, etc. Interested candidates should send CV/cover letter to RadOncTechnician@gmail.com and also apply via this posting. We look forward to hearing from you!

Choose Duke.

Anticipated Pay Range:

Duke University provides an annual base salary range for this position as USD $19.06 to USD $28.85. Duke University considers factors such as (but not limited to) scope and responsibilities of the position; candidate's work experience, education/training, and key skills; internal peer equity; as well as market and organizational considerations when extending an offer.

Your total compensation goes beyond the dollars on your paycheck. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array of family- friendly and cultural programs to eligible team members. Learn more at: https://hr.duke.edu/benefits/

Education

Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Degrees, Licensures, Certifications

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE