RESEARCH TECHNICIAN II
Durham, NC, US, 27710
School of Medicine
Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.
Occupational Summary
Perform a variety of complex technical duties involved in conducting laboratory tests, experiments and determinations in support of Duke University research operations.
Work Performed
- Setting up and operating laboratory equipment required for specific experiments; and preparing and maintaining solutions and other reagents for laboratory operations. Carrying out experiments using a variety of standard molecular and cell biology studies and histology approaches including, but not limited to, protein and nucleic acid purification, quantification, and analysis; plasmid DNA generation, manipulation, and analysis; bacteria and mammalian cell culture and analysis; mouse and human tissue harvest and analysis using histological approaches and molecular biology assays. Maintaining a laboratory notebook with accurate and timely documentation of laboratory work; meeting with the laboratory PI, as needed, to review data and progress and plan future research experimental priorities. Where appropriate, preparing or assisting with preparation of publication-quality figures or written reports for presentation or scientific publication.
- Assisting with the management of the laboratory mouse colony, including maintaining colony census and record of animal use; obtaining tissue for experimental analyses, including PCR-based genotyping.
- Assisting with maintaining day-to-day laboratory operations, including monitoring and replenishing supplies and reagents; cleaning and maintaining laboratory equipment; and assisting PI in preparing documents for institutional reporting, such as in maintaining IRB or IACUC protocols or biological/chemical safety protocols.
- Reviewing work of less experienced laboratory personnel, such as undergraduate students, within designated areas, and assist in training new personnel.
Required Qualifications at this Level
Education/Training:
Work generally requires a bachelor's degree in botany, biology, chemistry, or another related scientific field.
Experience:
None required above education/training requirement.
Skills:
Skills: Experience from work or laboratory-based educational courses in pertinent molecular and/or cell biology assays, such as general biology/chemistry knowledge. A combination of some or all of the following: nucleic acid and protein extraction and measurement; plasmid DNA preparation, manipulation, and analysis; polymerase chain reaction (PCR); agarose and/or SDS-polyacrylamide gel electrophoresis and blotting; mammalian and/or bacterial cell culture and analysis; fluorescence and light microscopy; experience with personal computer hardware and software for word processing, data storage and analysis, digital image processing, and scientific literature searches. Familiarity with molecular biology,
skeletal muscle development, and/or cancer biology are preferred.
Anticipated Pay Range:
Duke University provides an annual base salary range for this position as USD $18.69 to USD $28.28. Duke University considers factors such as (but not limited to) the scope and responsibilities of the position, the candidate's work experience, education/training, and key skills, internal peer equity, as well as market and organizational considerations when extending an offer.
Your total compensation goes beyond the dollars on your paycheck. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array of family-friendly and cultural programs to eligible team members. Learn more at: https://hr.duke.edu/benefits/
Duke is an Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex (including pregnancy and pregnancy related conditions), sexual orientation or military status.
Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends onthe robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.
Nearest Major Market: Durham
Nearest Secondary Market: Raleigh