RESEARCH TECHNICIAN II

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

Position Summary:

The Department of Radiation Oncology at Duke University is seeking a highly motivated and detail-oriented Research Technician to join our research team. The successful candidate will play a central role in managing day-to-day operations of a dynamic laboratory and supporting research efforts in cancer biology and radiation science. This position offers the opportunity to contribute to cutting-edge translational research in a collaborative academic environment.

Key Responsibilities:

Laboratory Management and Operations

· Oversee daily lab operations and maintain an organized, efficient laboratory environment

· Manage laboratory inventory, ordering, and receiving of supplies and reagents

· Ensure proper functioning and maintenance of laboratory equipment

· Enforce safety and compliance protocols in accordance with institutional guidelines

Cell Culture and Molecular Biology

· Maintain and passage mammalian cell lines under sterile conditions

· Perform routine cancer biology and molecular biology techniques including:

o DNA/RNA/protein extraction and quantification

o PCR, RT-PCR, and quantitative PCR

o Gel electrophoresis and western blotting

o Plasmid cloning, bacterial transformation, and miniprep/maxiprep

o Cell infection/transfection/transduction

o Cryopreservation and revival of cell lines

o Cell counting and viability assays

o Flow cytometry and basic cell sorting procedures

Animal Colony Management

· Maintain a small mouse colony:

o Set up breeding cages and monitor litters

o Perform tail clipping, tattooing, and genotype progeny

o Wean and organize litters for experiments

· Maintain accurate and up-to-date records of animal inventory, breeding, and experimental use in accordance with IACUC protocols

Required Qualifications:

· Bachelor’s degree in biology, biochemistry, molecular biology, or a related field

· At least 1–2 years of prior hands-on laboratory experience, preferably in an academic or research setting

· Familiarity with aseptic technique, mammalian cell culture, and molecular biology protocols

· Experience working with laboratory animals (mice) is strongly preferred

· Excellent organizational skills and attention to detail

· Ability to work independently and as part of a collaborative research team

· Strong communication skills and the ability to maintain accurate, timely, and thorough documentation

Anticipated Pay Range:

Duke University provides an annual base salary range for this position as USD $18.69 to USD $28.28. Duke University considers factors such as (but not limited to) the scope and responsibilities of the position, the candidate's work experience, education/training, and key skills, internal peer equity, as well as market and organizational considerations when extending an offer.

Your total compensation goes beyond the dollars on your paycheck. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array of family-friendly and cultural programs to eligible team members. Learn more at: https://hr.duke.edu/benefits
 

Duke is an Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex (including pregnancy and pregnancy related conditions), sexual orientation or military status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends onthe robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.