Duke University and Duke University Health System require all students, faculty and staff to receive the COVID-19 and influenza vaccines.  After you have officially accepted a position at Duke, you may request a religious or medical accommodation.


Work Arrangement: 
Requisition Number:  230319
Regular or Temporary:  Regular

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER
Date:  May 20, 2023
School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.



The Duke Human Vaccine Institute (DHVI), an international leader in the fight against major infectious diseases, is seeking a highly motivated individual for the position of Research Technician II. Investigators at the DHVI conduct basic and translational research to develop novel vaccines, therapeutics and diagnostics.

The Duke Regional Biocontainment Laboratory (RBL) is a division of the DHVI and supports a wide portfolio of research programs. The RBL has three collaborative core units: Immunology, Virology, and Microbiology. The Research Technician will work with the RBL Immunology team to prepare specimens for flow cytometry, PBMC isolation, ELISA, Luminex and other immunological assays. In addition, the technician will collect and interpret data and perform general laboratory maintenance and inventory management.

The ideal candidate will have a Bachelor’s degree in biological science or a related field.  Previous laboratory experience in immunology, microbiology, virology and/or molecular biology is strongly preferred. Experience with flow cytometry, molecular biology, laboratory automation and/or ELISAs preferred but not required.


  • Prepare specimens for and perform immunological procedures, including but not limited to: flow cytometry, PBMC isolation, ELISA and Luminex assays.
  • General laboratory maintenance including but not limited to: laboratory organization, preparation of solutions, waste processing, ordering/preparation/stocking of consumables and reagents, routine equipment calibration, maintenance of accurate sample and equipment inventories.
  • Coordinate equipment maintenance performed by outside vendors.
  • Attend all required training.
  • Confer with Immunology Unit Manager / Principal Investigator to review work assignments and develop plans for experiments; make minor modifications to existing laboratory procedures and techniques to meet the needs of particular studies as required.
  • Manage and complete all assigned tasks/projects in accordance with standardized operating procedures and in full compliance with all safety protocols.
  • Complete and maintain written and electronic documentation of research activities in real time according to standardized record keeping procedures.
  • Collect and organize raw data into reports for interpretation and analysis.
  • Perform additional duties as requested by Immunology Unit Manager and RBL/DHVI leadership.



  • Excellent interpersonal, problem-solving, communication and organizational skills. 
  • Flexibility, motivation, and ability to work independently. 
  • Willingness and ability to work on multiple projects simultaneously. 
  • Analytical skills to resolve problems requiring the use of scientific, mathematical, or technical principles.
  • Must be focused, driven, detail-oriented, well organized with strong communication skills and able to work in a team environment.


The successful applicant must be eligible and willing to:

  • Complete necessary training and certification for independent work at Biological Safety Level 3 (BSL3).
  • Work with infectious biological agents (viruses and bacteria) handled at BSL2 and BSL3.
  • Comply with vaccination requirements.
  • Complete Tier 1 Select Agent registration.










Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

  • Complete necessary tr aining and certification for independent work at Biological SafetyLevel 3 (BSL3).
  • Work with infectious biological agents (viruses and bacteria) handled at BSL2 andBSL3.
  • Comply with vaccination requirements.
  • Complete Tier 1 Select Agent registration.


Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh