Share this Job

 
Duke University and Duke University Health System require all students, faculty and staff to receive the COVID-19 and influenza vaccines.  After you have officially accepted a position at Duke, you may request a religious or medical accommodation.

POSTDOCTORAL ASSOCIATE

Work Arrangement: 
Requisition Number:  197142
Regular or Temporary:  Regular
Location: 

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER
Date:  Aug 6, 2022

This Position is Grant Funded

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

 

 

POSTDOCTORAL ASSOCIATE OPPORTUNITY

 

The Duke Human Vaccine Institute (DHVI) performs basic and translational research to fight against major infectious diseases. DHVI is seeking Postdoctoral Associates to join our team in developing vaccines for HIV and beta-coronaviruses such as SARS-CoV-2.  The Duke Human Vaccine Institute is an interdisciplinary, interdepartmental institute dedicated to the study of host-pathogen interactions that can be translated into biologics and vaccines. DHVI is composed of a team of highly interactive investigators that have expertise in mucosal and systemic immunology, virology, molecular and structural biology, computational biology, and animal medicine. With these expertises, the DHVI is working collaboratoratively to develop vaccines and countermeasures against pathogens like HIV, SARS-CoV-2, and influenza.

 

We are seeking a highly motivated recent graduate to join our team investigating the development of a pan-betacoronavirus vaccine. The investigation of the neutralizing antibody repertoire from SARS-CoV-2 spike-vaccinated nonhuman primates will be a central responsibility of the Postdoctoral Associate. The laboratory investigates the broadly reactive antibody response to betacoronaviruses following vaccination with SARS-CoV-2 spike multimerized nanoparticles. Many of the vaccine-induced antibodies neutralize other betacoronaviruses, such as SARS-CoV-1, SARS-CoV-2, batCoV-SHC014, and BatCoV-WIV1.  We utilize molecular insights of how neutralizing antibodies interact with different coronavirus spike proteins to design potential multivalent immunogens. The Postdoctoral Associate will work independently to perform immunoassays, fluorescence-activated single B cell sorting, and antibody cloning. More specifically, they will characterize the binding and neutralization phenotypes of the monoclonal antibodies identified by cell sorting and next generation sequencing. The Postdoctoral Associate will also investigate the ontogeny of vaccine-induced broadly reactive antibodies. Under the guidance of the Principal investigator, the candidate will be responsible for designing and executing experiments, interpreting the data, and presenting the results at internal and external meetings and in scientific publications.

 

The Postdoctoral Associate will perform some or all of the following activities:

  • Fluorescence-activated cell sorting of single B cells from spike-vaccinated macaques
  • PCR amplification of antibody genes
  • Expression of recombinant SARS-CoV-2 antibodies and immunogens by transient transfection
  • Illumina MiSeq next generation sequencing of antibody genes
  • Antibody sequences analysis
  • Design, expression, and purification of coronavirus protein immunogens for use in immunoassays
  • Immunoassays to include luminex, ELISA, western blots, and biolayer interferometry
  • Present scientific results at meetings (internal and external) and publish results in peer-reviewed journals
  • Training for bioethics, laboratory management, and scientific writing
  • Maintain accurate laboratory documentation of experiments, including raw experimental data and laboratory notebooks

The position requires a PhD in biological sciences, chemistry, or a related discipline and a strong record of publications in peer-reviewed journals. Ideal candidates will possess excellent oral and written communication skills with the ability to work in a highly collaborative team environment.

 

 

 

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

 

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

 

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

 


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh