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Req ID:  1894
Date:  Nov 7, 2019

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER

School of Medicine:


Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.


Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.


The Duke Human Vaccine Institute and the Center for HIV/AIDS Vaccine Immunology & Immunogen Design (CHAVI-ID), providing national and international leadership in the fight against major infectious diseases, is currently recruiting for a Postdoctoral Associate positions. The Duke Human Vaccine Institute (DHVI) is an interdisciplinary, interdepartmental institute dedicated to the study of basic and translational science required to understand host-pathogen interactions that can be translated to vaccines against human diseases. DHVI comprises a team of highly interactive investigators that have expertise in mucosal and systemic virology, molecular biology, microbiology and animal models.


We are seeking a highly motivated recent graduate to join our teaminvolved in the assessment of Vaccine Induced HIV-1 Env Antibody Responses. The most efficacious HIV-1 vaccine to date elicited non- neutralizing antibody responses whose titers inversely correlated with infection risk. Additionally, the non- neutralizing antibody effector function, antibody-dependent cellular cytotoxicity (ADCC) inversely correlated with infection risk. Therefore, vaccines that elicit high titers of ADCC-mediating antibodies are of interest. The Postdoctoral Associate will work as partof a team to design novel mRNA vaccines to elicit protective HIV-1 antibody responses. The candidate will compare immunogens produced from the mRNA platform to other vaccine platforms such as recombinant protein. The Postdoctoral Associate will work independently to isolate and characterize HIV-1 antibodies from vaccinated guinea pigs and nonhuman primates to discern whether vaccine- induced antibodies target known ADCC epitopes. Additionally, the Postdoctoral Associate will work independently to performimmunoassays characterizing the functions of the antibodies. These assays will include ELISA, luminex, phagocytosis, ADCC and complement fixation assays for serum polyclonal antibodies as well as monoclonal antibodies isolated from vaccinated animals. Under the guidance of the Principal investigator, the candidate will be responsible for designing and executing experiments, interpreting the data, presenting the results at internal and external meetings, and drafting scientific publications.


More specifically, the Postdoctoral Associate with perform the following activities:

• Transient transfection of mRNA and DNA envelope constructs to evaluate expression.

• Antigenic characterization of HIV-1 envelope by flow cytometry and ELISA

• Design, expression,and purification of HIV-1 envelopes for use in immunoassays.

• Fluorescence-activated cell sorting of single B cells from vaccinated animals.

• PCR amplification of antibody genes.

• Expression of recombinant HIV-1 antibodies by transient transfection.

• Illumina MiSeq next generation sequencing of antibody genes.

• Antibody sequences analysis. • Immunoassays to include (luminex, ELISA, and Western Blots).

• Present scientific results at meetings (internal and external) and publish results in peer-reviewed journals.

• Participation in training opportunities for bioethics, laboratory management, and scientific writing.

• Maintain accurate laboratory documentation of experiments, including raw experimental data and laboratory notebooks.



The position requires a MD or PhD in biological sciences, chemistry, or a related discipline 


This position requires a strong record of publications in peer-reviewed journals. Ideal candidates will possess excellent oral and written communication skills with the ability to work in a highly collaborative team environment.


Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh