LABORATORY RESEARCH ANALYST I

Work Arrangement:

Requisition Number: 251897

Regular or Temporary: Regular

Location:

Durham, NC, US, 27710

Personnel Area: MEDICAL CENTER

Date: Dec 17, 2024

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

Olabisi Lab Job Description:
LAB RESEARCH ANALYST I

Occupational Summary

The applicant will be part of the OLABISI lab, a physician–scientist run lab that studies how APOL1 gene variants cause kidney disease in individuals of recent African ancestry. APOL1 Kidney disease explain 40% of excess risk of kidney failure in African Americans. The lab leverages human derived cell-based models and APOL1-transgenic mouse models to study the underlying mechanism of this devastating disease. The goal of the lab is to discover innovative prevention and cure APOL1 Kidney disease. Olabisi Lab is a vibrant research environment that interacts with other researchers in Duke Molecular Physiology Institute and Duke Division of Nephrology.

Work Performed

Perform DNA genotyping by Taqman Assay and Sanger sequencing
Manage a transgenic mouse colony, including but not limited to live mouse handling, ordering, genotyping, breeding, routine monitoring of animal health, sample collection and processing, maintaining and updating mouse line records etc.
Assist in the design and analysis of experiments; carry out experiments with minimal or no immediate supervision and record and maintain all acquired data in specified formats for scientific reliability and general comprehension
Administering compounds to mice (injections, oral gavage), collecting urine and blood, performing dissections to isolate and characterize various tissues from mice.
Process mouse biospecimens such as urine, blood and tissues for flow ELISA, histology, Immunoblotting, nucleic acid isolation etc.
Perform simple techniques in cell biology and microscopy: examples include cell isolation and culture procedures, immunocytochemistry, tissue cryosectioning, microscope imaging, cell sorting, and some basic molecular biology and biochemistry.
Maintain cell culture lines, including preparation of culture media, chemicals, reagents and solutions
Perform RNA extraction and qPCR
Perform routine lab maintenance, cleaning, ordering, and organizing.
Maintain lab SOPs, maintain organization of the lab which includes all equipment, freezers, fridges, reagents in these areas and general upkeep of lab space.
Understand, comply and assist in enforcing all safety standards and procedures to ensure a safe working environment for all laboratory staff
Maintain adequate stock of lab supplies and ensure proper setup and functioning of equipment.
Maintain detailed and accurate documentation of activities in an electronic laboratory notebook.
Learn and develop new lab techniques and assist in the training of new personnel.
Perform job-related tasks other than those specifically presented in this description.

The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.

Required Qualifications at this Level

Education/Training:	Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience:	Work requires two years of research experience. A related master's degree may offset the required years of experience on a 1:1 basis, e.g., a two-year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Preferred Education and Skills:	Excellent interpersonal, problem-solving, communication and organizational skills. Proficiency in Experience with the care and handling of mice, proficiency in collecting urine and blood from mice, and molecular biology techniques is preferred, ideally with some experience outside of laboratory classwork (i.e. in a working laboratory) Analytical skills to resolve complex problems requiring the use of scientific, technical principles and in-depth, experienced-based knowledge Flexibility, motivation, and ability to work independently. Willingness and ability to take on multiple projects simultaneously.

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.