LAB RESEARCH ANALYST II

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

The Moseman laboratory at Duke University is seeking a detail-oriented individual for the position of Lab Research Analyst II to join our team.  The Moseman lab uses a variety of infectious pathogens together with transgenic mouse models, flow cytometry, confocal and intravital imaging, and transcriptomic analyses to study host:pathogen interactions within the upper airway and brain.  Read more about our interests at https://www.mosemanlab.com/

Responsibilities

• Manage a transgenic mouse colony, including but not limited to: live mouse handling, ordering, genotyping, breeding, routine monitoring of animal health, sample collection and processing, maintaining and updating mouse line records etc.
• Assist in the design and analysis of experiments; carry out experiments with minimal or no immediate supervision and record and maintain all acquired data in specified formats for scientific reliability and general comprehension
• Administering compounds to mice (injections, oral gavage), performing dissections to isolate and characterize lymphocytes from various tissues, and conducting relevant animal models of disease (e.g., infections and immunizations)
• Process mouse biospecimens such as blood and tissues for flow cytometry, histology, viral and antibody titering, DNA, RNA, etc. Perform simple techniques in cell biology and microscopy: examples include cell isolation and culture procedures, immunocytochemistry, tissue cryosectioning, microscope imaging, cell sorting, and some basic molecular biology and biochemistry.
• Maintain cell culture lines, including preparation of culture media, chemicals, reagents and solutions
• Perform routine lab maintenance, cleaning, ordering, and organizing.
• Maintain lab SOPs, maintain organization of the lab which includes all equipment, freezers, fridges, reagents in these areas and general upkeep of lab space.
• Understand, comply and assist in enforcing all safety standards and procedures to ensure a safe working environment for all laboratory staff
• Maintain adequate stock of lab supplies and ensure proper setup and functioning of equipment.
• Maintain detailed and accurate documentation of activities in an electronic laboratory notebook.
• Learn and develop new lab techniques and assist in the training of new personnel.
• Employee may be directed to perform job-related tasks other than those specifically presented in this description. 

Preferred Skills: 

• Excellent interpersonal, problem-solving, communication and organizational skills. 
• Experience with the care and handling of mice, flow cytometry, and molecular biology techniques is preferred, ideally with some experience outside of laboratory classwork (i.e. in a working laboratory) 
•  Analytical skills to resolve complex problems requiring the use of scientific, mathematical, or technical principles and in-depth, experienced-based knowledge
• Flexibility, motivation, and ability to work independently. 
• Willingness and ability to take on multiple projects simultaneously.

Minimum Qualifications

Education

Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

Work requires four years of research experience. A related master's OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.