Work Arrangement: 
Requisition Number:  240880
Regular or Temporary:  Regular

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER
Date:  Feb 17, 2024
School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Lab Research Analyst II

DHVI Genomics Core


Position Overview:


The Duke Human Vaccine Institute (DHVI) is internationally recognized for its research and development of vaccines and therapeutics targeting HIV and other emerging infections that threaten the health of our nation and our world. The DHVI Genomics Core serves the needs of the DHVI, Duke Center for AIDS Research, Duke University and external collaborators by providing high-throughput genomic technologies and services including sequencing on Illumina platforms, preparation of genomic and transcriptomic sequencing libraries, single cell library preparation using the 10X Genomics platform, automated amplicon purification, as well as DNA/RNA isolation, purification, and quality assessment. We also specialize in bulk and single-cell profiling of immune repertoires. Our goal is to provide the highest quality service to our investigators at all stages of their studies including experimental design, sample preparation, quality control, and analysis.

The DHVI Genomics Core is hiring a Laboratory Research Analyst II. The successful candidate for this position will conduct a variety of complex SOP-driven and independent activities including experimental design, technology development, project management, and data analysis for studies performed within the core. The candidate will correspond and collaborate with researchers and scientists from multiple disciplines across the DHVI and Duke’s campus. The candidate will operate technical laboratory instruments and equipment such as automated liquid handlers and high throughput sequencers. The candidate will conduct multiple projects concurrently, requiring excellent organizational and time management skills to perform experiments effectively within specified deadlines. This position reports to the Genomics Core Director.

To apply, please send a cover letter, CV, relevant publications if applicable, and names of three references. In the cover letter, the applicant should describe their research experience. We highly encourage candidates that identify with a marginalized or underrepresented group to apply.


Work Performed


Develop, onboard, and execute single-cell sequencing and functional genomic assays. Independently schedule and plan experimental activities to efficiently deliver data. Troubleshoot experiments with teammates and provide technical support when necessary. Standardize new protocols and train teammates on new techniques and assays.

Perform routine laboratory tasks including but not limited to NGS high-throughput library preparations, quality control of samples and libraries, nucleic acid extraction, pooling and diluting samples, and loading sequencers.

Correspond with principal investigators and researchers to provide troubleshooting and guidance on sequencing protocols and logistics assistance.

Document and adhere to standardized laboratory methods, including development and updating of SOP’s regarding experimental methods.

Review lab methods, manuals, abstracts, and other literature for information applicable to ongoing research experiments.

Assist with laboratory operational and administrative tasks (e.g. sample accession, order updates, machine maintenance, etc.)

The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.


Necessary skills and abilities:

  • Experience with single-cell genomics
  • Proficiency in the development of NGS methods and complex molecular biology assays
  • Strong analytical skills and scientific understanding of NGS protocols
  • Outstanding written and oral communication skills
  • Excellent problem-solver both scientifically and operationally
  • Works well both independently and collaboratively
  • Ability to prioritize and efficiently complete multiple tasks and projects
  • Ability to establish and maintain working relationships with administration, clients, and the core facility staff
  • Basic computer skills (e.g. spreadsheet and word processing software)


Required Qualifications at this Level:


  • Education/Training: Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
  • Experience: Work requires four years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two-year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE.



  • Ph.D. in genetics, genomics, biology, molecular biology, immunology or related field
  • Hands on experience generating multiple types of next-generation sequencing data (WGS, WES, RNA-seq, ATAC-seq, etc.)
  • Experience with 10x Genomics platforms is preferred.
  • Experience with Illumina sequencing platforms is preferred.
  • Publications and notable significant contributions to scientific research is a plus.
  • Programming and advanced statistical skills (Linux, Python, R)


Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh