LAB RESEARCH ANALYST II

Duke University:

Duke University was created in 1924 through an indenture of trust by James Buchanan Duke. Today, Duke is regarded as one of America’s leading research universities. Located in Durham, North Carolina, Duke is positioned in the heart of the Research Triangle, which is ranked annually as one of the best places in the country to work and live. Duke has more than 15,000 students who study and conduct research in its 10 undergraduate, graduate and professional schools. With about 40,000 employees, Duke is the third largest private employer in North Carolina, and it now has international programs in more than 150 countries.

The Gerecht lab (https://pratt.duke.edu/faculty/sharon-gerecht) is looking for a highly motivated individual to join our interdisciplinary research program at the Department of Biomedical Engineering at Duke University. Our vascular tissue engineering lab combines cutting-edge techniques from various disciplines, including stem cells, vascular biology, biomaterials, mechanobiology, cellular and molecular biology, etc. 

The Associate in Research will be part of a collaborative team to establish and implement research protocols for complex and/or multiple research studies with a focus on cell and molecular biology. Provide scientific, training, grants, and reporting support and other research responsibilities involving the research activities of the lab. As part of a study team, collaborate with trainees, develop protocols, and supervise study-related activities for one or more studies. 

Specific Duties & Responsibilities: 

Experimental Support:

•Independently conduct laboratory-based experiments. These assays will mainly focus on gene editing of stem cells and their derivatives using CRISPR and other methods for generating stable cell lines. These will include but are not limited to: tissue and mammalian cell culture, cloning, gene expression analysis, immunohistochemistry, immunofluorescence microscopy, flow cytometry, ELISA, protein arrays.

• Teach assays to other laboratory personnel and collaborate with personnel in laboratory-based experiments and assays for the various research projects 

• May also provide support for animal studies in the lab including animal handling and maintenance, preparation for surgeries, occasional measurements, etc 

• Interpret data, write methods and protocols. 

Reporting: 

• In collaboration with the PI, track activities, milestones, and deliverables. 

• Along with the PI, provide support and submit progress reports for research activities.

Laboratory management:

• Coordinating and maintaining certifications for lab equipment and personnel-related lab trainings.
• Overseeing and managing the completion of required lab safety trainings, records and compliance of lab personnel.
• Record keeping for orders/receipts, lab safety roster, key logs, protocols, safety certifications, equipment warranty, etc. (including lists of cell lines and lab chemicals)
• Coordinating lab meetings, coordinate ordering of supplies with lab personnel, coordinate and plan diverse lab-related events                   

Education/experience:

Master's degree in a related discipline or Bachelor's degree in bioengineering, biology, chemistry, or related field with at least two years of related experience. Experience with genetic manipulation of mammalian cells and cloning is desirable. Additional education may substitute for required experience, to the extent permitted by the Duke equivalency formula.

Special Skills and Knowledge:

Ability to work independently, multi-task and to effectively prioritize workload and be proactive in order to meet deadlines.  Ability to work collaboratively as part of a team. Ability to work effectively with researchers from a variety of diverse backgrounds.

Minimum Qualifications

Education

Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

Work requires four years of research experience. A related master's OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.