LAB RESEARCH ANALYST I

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

Position Description:

Our research focuses on determining the molecular and cellular mechanisms by which procoagulant platelets and endothelial cells regulate thrombin formation. An understanding of these surface-dependent interactions is critical to our understanding of normal hemostasis and pathologic thrombosis including the unique thrombotic complications seen with non-factor replacement therapies for patients with hemophilia and other bleeding disorders. Our goal is to utilize these findings to improve the care and quality of life for patients with bleeding and clotting disorders through preclinical studies of potential therapeutic agents.

We seek a highly motivated candidate for a laboratory research analyst (LRA1) position to perform cell culture, molecular biology, biochemistry, cell biology, and animal research using bleeding and thrombosis models. This candidate will work under the supervision of the Principal Investigator in the Division of Hematology. This position currently has funding for 1 year, but may be renewed based on available funding and performance.

Primary Responsibilities

· Perform essential cell culture work on human cells for in vitro and in vivo studies.

· Design and perform molecular assays, including but not limited to DNA/RNA isolation PCR, qPCR, SDS-PAGE gels, western blot, and ELISA.

· Obtain whole blood specimens by venipuncture from properly identified human subjects and provide properly prepared specimens for in vitro studies.

· Perform experiments in mice, including, but not limited to, breeding, handling, anesthesia, non-survival surgeries, intravenous and tail vein injections, weighing, and preparation of tissues for flow cytometry, histology, or RNA/DNA/protein isolation.

· Operate and maintain laboratory equipment required for specific tests.

· Prepare and maintain reagents, buffers, and media for experiments.

· Assist with managing IACUC protocols, lab safety SOPs, and lab ordering.

· Maintain detailed and complete records of all work performed in an organized lab notebook.

· Analyze, evaluate, and interpret data for communication of results to the PI and other lab members in team meetings.

· Must be detail oriented and well organized with strong communications skills and the ability to execute work independently as needed

· Meet with the PI regularly to plan experiments, troubleshoot problems, and discuss results.

· Review journals, abstracts, and scientific literature to obtain information to aid in the design and development of procedures and techniques.

· Help provide a collegial, supportive, and professional working environment

· Perform other related duties as required by the PI.

· Must be a US Citizen, National, or authorized to work in the US.

Required Qualifications at this Level

Education/Training

Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two-year master's degree in lieu of two years of experience.

OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes.To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Anticipated Pay Range:

Duke University provides an annual base salary range for this position as USD $50,439.00 to USD $76,417.00. Duke University considers factors such as (but not limited to) scope and responsibilities of the position; candidate's work experience, education/training, and key skills; internal peer equity; as well as market and organizational considerations when extending an offer.

Your total compensation goes beyond the dollars on your paycheck. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array of family-friendly and cultural programs to eligible team members. Learn more at: https://hr.duke.edu/benefits/

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/ or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Education

Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Duke is an Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex (including pregnancy and pregnancy related conditions), sexual orientation or military status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends onthe robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.