Work Arrangement: 
Requisition Number:  239363
Regular or Temporary:  Regular

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER
Date:  Feb 22, 2024

This Position is Grant Funded

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.


Macintyre Lab – Duke Human Vaccine Institute


The Duke Human Vaccine Institute (DHVI), an international leader in the fight against major infectious diseases, is seeking a highly motivated individual for the position of Laboratory Analyst I. Investigators at the DHVI conduct basic and translational research to develop novel vaccines, therapeutics and diagnostics. 


The Duke Regional Biocontainment Laboratory (RBL) is a division of the DHVI and supports a wide portfolio of research programs. The RBL has three collaborative core units: Immunology, Virology, and Microbiology. The Lab Research Analyst I will work with the RBL Immunology team and Duke Investigators to plan, run and analyze flow cytometry assays plus other immunoassays such as ELISpot and ELISA. 



  • Prepare samples for and perform flow cytometry (including techniques such as intracellular cytokine staining and multi-parameter surface marker staining). 
  • Analyze flow cytometry data using specialized software (e.g. FlowJo) and prepare tabular and graphical summaries for reporting to collaborators.  
  • Assist Investigators and Immunology Unit leadership with the design and optimization of flow cytometry staining panels. 
  • Prepare and process specimens for immunological procedures other than flow cytometry, including but not limited to ELISpot, Luminex and ELISA.  
  • Confer with Immunology Unit leadership to review work assignments and develop plans for experiments. 
  • Complete and maintain electronic documentation of research activities in real time.  
  • Manage and complete all assigned tasks/projects in accordance with standard operating procedures (SOP) and in full compliance with all safety protocols. 
  • Develop, write and maintain SOP. 
  • Attend all required training. 
  • General laboratory maintenance including but not limited to: laboratory organization, preparation of solutions, waste processing, ordering/preparation/stocking of consumables and reagents, routine equipment calibration, maintenance of accurate sample inventories. 
  • Perform additional duties as requested by Immunology Unit Leadership and RBL/DHVI leadership.          

The successful applicant must be eligible for and willing to: 

  • Complete necessary training and certification for independent work at Biological Safety Level 3 (BSL3). 
  • Work with infectious biological agents (viruses and bacteria) handled at BSL2 and BSL3. 
  • Comply with vaccination requirements. 
  • Complete Tier 1 Select Agent registration. 



  • Education/Training: Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
  • Experience: Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two-year master's degree in lieu of two years of experience. Or an equivalent combination of relevant education and/or experience.



  • Excellent interpersonal, problem-solving, communication and organizational skills.  
  • Ability to work independently and as part of an interdisciplinary team.  
  • Willingness and ability to work on multiple projects simultaneously.  
  • Analytical skills to resolve problems requiring the use of scientific, mathematical, or technical principles. 
  • Previous experience with flow cytometry is highly preferred. 


Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh