LAB RESEARCH ANALYST I

LAB RESEARCH ANALYST I 

Duke Regional Biocontainment Laboratory – Duke Human Vaccine Institute

The Duke Human Vaccine Institute (DHVI), is seeking a highly motivated Lab Research Analyst I to join our team studying thymus development and function. The Duke Regional Biocontainment Laboratory (RBL) is a division of the DHVI and supports a wide portfolio of research programs. The RBL has three collaborative core units: Immunology, Virology, and Microbiology. The Lab Research Analyst I will work with the RBL Immunology team and Duke Investigators to plan, run and analyze flow cytometry assays, Luminex assays, and other immunoassays such as ELISA.  

RESPONSIBILITIES: 

• Prepare specimens for and perform flow cytometry (including techniques such as intracellular staining and 10+ parameter surface marker analysis). 
• Analyze flow cytometry data using specialized software (FlowJo, ForeCyte, Prism) and prepare tabular and graphical summaries for reporting to collaborators. 
• Prepare and process specimens for immunological procedures, including but not limited to Luminex assays, ELISAs and single-cell sequencing.
• Confer with Immunology Unit leadership / Principal Investigator to review work assignments and develop plans for experiments.
• Complete and maintain electronic documentation of research activities in real time. 
• Manage and complete all assigned tasks/projects in accordance with standard operating procedures (SOP) and in full compliance with all safety protocols.
• Develop, write and maintain SOP. 
• Attend all required training.
• General laboratory maintenance including but not limited to: laboratory organization, preparation of solutions, waste processing, ordering/preparation/stocking of consumables and reagents, routine equipment calibration, maintenance of accurate sample and equipment inventories.
• Perform additional duties as requested by Immunology Unit Manager and RBL/DHVI leadership.  

REQUIREMENTS: 

• Work requires a bachelor’s degree in botany, biology, zoology, psychology, or other directly related scientific field.
• Work requires two years of research experience. A related master’s degree may offset required years of experience on a 1:1 basis, e.g., a two-year master’s degree in lieu of two years of experience.
• Previous experience with flow cytometry is highly preferred but not essential.  

• Excellent interpersonal, problem-solving, communication, troubleshooting and organizational skills. 
• Ability to work independently and as part of an interdisciplinary team. 
• Willingness and ability to work on multiple projects simultaneously. 
• Must be focused, detail-oriented, and well organized.
• The successful applicant must be eligible for and willing to: 
  • Complete necessary training and certification for independent work at Biological Safety Level 3 (BSL3). 
  • Work with infectious biological agents (viruses and bacteria) handled at BSL2 and BSL3. 
  • Comply with vaccination requirements.
  • Complete Tier 1 Select Agent registration. 

Minimum Qualifications

Education

Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

Work requires two years of research experience. A related master's OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE degree may offset requiredyears of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.

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