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GMP CELL LINE DEVELOPMENT SCIENTIST

Req ID:  6942
Date:  Oct 26, 2019
Location: 

Durham, NC, US, 27710

Personnel Area:  MEDICAL CENTER

School of Medicine:

 

Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

 

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

 

The Cell Line Development Scientist will lead initiatives to develop stable cell lines and cell pools to express novel envelope proteins to support GMP manufacturing of potential vaccine candidates.  This position will conduct and document laboratory experiments which trace the generation of a sequence from receipt to incorporation into an expression vector and optimization for expression in a cell platform which is used to express GMP compliant recombinant vaccine candidates.  

 

Responsibilities

 

  • Lead the development of cell lines and efforts to identify optimal pools or lines which express recombinant vaccine meeting specifications determined by research and development teams within the DHVI as well as our collaborators.
  • Contribute new approaches to the development of methods for protein expression.
  • Perform transfection of mammalian cells using expression vectors engineered to express novel vaccine candidates.
  • Perform cell culture and passage in selection media to eliminate low and non-expressing cell populations.
  • Monitor cell pools and cell lines for cell density, viability and doubling time.  Interpret those results to determine sub-culture times and future strategy to optimize culture growth and expression.
  • Determine and optimize cell culture media and feeding strategies for long-term cultures.
  • Derive and characterize stable cell lines.
  • Sort cell populations using a cell sorter and sorting software.
  • Perform limiting dilution cloning to establish cell clones.  Operate CloneSelect and ClonePix to identify individual cell populations.
  • Develop collaborations with external organizations and provide support for development activities.  Work closely with analytical development functions to understand how the expressed product impacts product quality.
  • Maintains detailed records carefully documenting all process steps and reagents used under good documentation practices.  Maintains required laboratory supplies, reagents and consumables by ordering and tracking inventory.
  • Oversees and directs production and manufacture of master cell banks either in-house or by contract research organizations.
  • Directs release and characterization testing of master cell banks, including testing for adventitious virus testing to ensure compliance with FDA and EU standards.
  • Analysis and interpretation of data.  Assess and draw conclusions regarding progress and results of work, utilizing the data in appropriate decision making.
  • Mentors others in developing scientific skills and methods and may manage staff.
  • Perform other duties as assigned by DHVI leadership.

 

 

Minimum Qualifications

 

 

Education

Bachelor's degree in a science, engineering, or related field. An advanced degree (Master's, PhD) is preferred.

 

Experience

 

6 years relevant experience in the biopharmaceutical industry or equivalent.

 

Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

 

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

 

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

 


Nearest Major Market: Durham
Nearest Secondary Market: Raleigh