FLOW CYTOMETRY ENGINEER

This Position is Grant Funded

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Flow Cytometry Engineer

Flow Cytometry and Cell Sorting Shared Resource Facility – Duke Human Vaccine Institute

OVERVIEW:

The DHVI Flow Cytometry Facility has been providing state-of-the-art flow-based analysis and cell sorting for over 25 years. The facility is housed on the Duke West Campus and on the newly renovated Duke RTP Campus. The facility currently has three sorters (two five-laser BD FACSymphony S6s and a four-laser BD FACSAria II). Also, the facility currently has six analytical cytometers (a five-laser BD FACSymphony A5, a four-laser BD LSRFortessa, a four-laser BD LSRII, a three-laser IntelliCyt iQue Screener Plus, and two MilliporeSigma Muses). The facility supports over 120 principal investigators, provides comprehensive training for 24/7 independent use of analytical instruments, and sorts samples at biosafety levels 1, 2 and 3, including CDC Select Agents. The Flow Cytometry Engineer position will be responsible for maintaining and operating cytometers, and implementing innovative cytometry-based approaches in support of the scientific research activities of the Duke Human Vaccine Institute and collaborating entities. 

RESPONSIBILITIES: 

• Fully comply with appropriate DHVI/GHRB Safety and Compliance SOPs.  
• Run cell sorters/analyzers to meet the needs of all facility end users, as directed by the Facility Director, Manager and DHVI leadership. 
• Perform regular preventative maintenance, repairs, and upgrades on sorters and analyzers.  
• Work with DHVI Flow Facility Director/Manager in development of new flow cytometric technologies and applications. 
• Ensure data, run templates, sort reports and all associated documents/files per end user and facility SOPs are uploaded into appropriate facility archival systems. 
• Maintain Select Agent Authorization and BSL-3 work certification.  
• Perform other duties as requested by the DHVI Flow Facility Director/Manager, Shared Resources Scientific Director, COO and Director. 

WORK HOURS:   

You must be willing and available to work flexible hours which may include later start and end times with the understanding that work hours may need to be extended until a project/task has reached a logical and acceptable stopping point to maintain the integrity of the science. You also must be willing to travel between and work at both main campus and RTP laboratories. 

REQUIREMENTS:   

• Bachelor’s degree in science related field or electronic technology related field. Four years of military technical training and/or vocational training in electronics or technology may substitute for the degree requirement. 
• Minimum of 5 years of direct relevant experience.

Willing and able to: 

• Work with hazardous biological agents handled at BSL2 and BSL3.
• Comply with vaccination requirements when necessary.
• Complete US Department of Justice screening for Select Agent registration. 
• Complete Select Agent registration at Tier 1. 
• Achieve external certification for independent work at BSL3.

DESIRED SKILLS: 

• Experience in maintenance/repair and operation of BD FACS Aria II and/or other BD flow cytometers. 
• Experience in BD cytometry software, including DIVA. 
• Experience in Biosafety Levels 2 and 3 containment.

Minimum Qualifications

Education

Bachelor's degree in science related field or electronic technology related field. Four years of military technical training and/or vocational training in electronics or technology may substitute for the degree requirement. 2. Minimum of 5 years of direct relevant experience 3. Willing and able to: a. Work with hazardous biological agents handled at BSL2 and BSL3 b. Comply with vaccination requirementswhen necessary c. Complete US Department of Justice screening for Select Agent registration d. Complete Select Agent registration at Tier 1 e. Achieve external certification for independent work at BSL3

Experience

Minimum of 5 years of direct relevant experience

Degrees, Licensures, Certifications

Comply with vaccination requirements when necessary Complete US Department of Justice screening for Select Agent registration Complete Select Agent registration at Tier 1 Achieve external certification for independent work at BSL3

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.